The NAD+ triple, with the NAD+ done right. No more sprinkle.
Our NAD+ + MOTS-c + 5-Amino-1MQ blend is one of the most popular vials we make, and it is worth calling it what it is: a MOTS-c and 5-Amino-1MQ blend with a little NAD+ on top. IGNITE-540 is the version where all three lead. 500mg of NAD+, 20mg of MOTS-c, 20mg of 5-Amino-1MQ. Five times the NAD+, double the other two, one vial, every component weighed on the certificate.
The fuel. The signal. The guard. All three at weight.
Nicotinamide adenine dinucleotide. The coenzyme every redox reaction in energy metabolism runs on, and the substrate sirtuins and PARPs consume.
A 16-amino-acid peptide encoded in mitochondrial DNA. Researched as an AMPK-activating, exercise-mimetic metabolic regulator.
A small-molecule NNMT inhibitor. Studied for keeping nicotinamide in the NAD+ salvage pathway instead of letting it be methylated away.
The three compounds sit on one system. NAD+ is the pool the cell draws on. 5-Amino-1MQ blocks the enzyme that drains that pool. MOTS-c is the signal that puts the pool to work. In the 100/10/10 the guard and the signal were at full weight while the pool they serve was a garnish. IGNITE-540 fills the pool.
| Component | 100/10/10 | IGNITE-540 |
|---|---|---|
| NAD+ the fuel | 100 | 500 |
| MOTS-c the signal | 10 | 20 |
| 5-Amino-1MQ the guard | 10 | 20 |
| Total · ratio | 120 · 10:1:1 | 540 · 25:1:1 |
Nothing was taken out to make room. Both leads doubled and the NAD+ went up five-fold, so the only thing that changed about the balance is that NAD+ stopped being the smallest part of its own blend. The 100/10/10 stays in the catalog for the MOTS-c and NNMT question it was always good at.
Ten vials at 40% off run $720, which is $72 a vial and $0.13 per milligram of blended material, with 500mg of NAD+ in every one. The ladder is 2 vials 5%, 3+ vials 10%, 5-pack 25%, 10-pack 40%, off the single-vial price, applied in the cart. Any mixed box of ten vials across products takes 25% off.
Build the same 540mg from the singles and you spend $165, reconstitute five vials, draw from three of them every time, and bank half a NAD+ vial you did not need. Buy IGNITE-540 and you spend $120 for the identical material in one vial, one reconstitution, one draw, with the split weighed line by line on the certificate. That is 27% less, and a 10-pack at $72 a vial is less than half the singles route.
Why This Ratio
NAD+ is not a peptide and it is not used like one. It is a coenzyme the cell turns over continuously, and NAD+ research runs it in the hundreds of milligrams per administration. Our NAD+ single is a 1000mg vial for that reason. MOTS-c and 5-Amino-1MQ, by contrast, are studied in the low milligrams. Put the three in one vial at 100/10/10 and the two small compounds land at a workable weight while the NAD+ is a tenth of its own vial, which is why that blend behaves like a MOTS-c and 5-Amino-1MQ blend with NAD+ along for the ride. It is a good blend for that question. It just is not an NAD+ blend.
IGNITE-540 fixes the one number that was off and leaves the rest alone. 500mg of NAD+ is half the single, inside the range NAD+ is studied at by itself, so NAD+ becomes a full partner. 20mg each of MOTS-c and 5-Amino-1MQ doubles the leads, so the move from 10:1:1 to 25:1:1 comes entirely from NAD+ going up rather than from the small components going down. There is also a mechanistic reason to want the NAD+ at weight: 5-Amino-1MQ only protects an NAD+ pool if there is a pool to protect, and MOTS-c only has fuel to signal over if the fuel is there.
What Each Compound Brings
NAD+ (nicotinamide adenine dinucleotide) is the coenzyme that shuttles electrons through glycolysis, the TCA cycle and the electron transport chain, and the substrate consumed by sirtuins, PARPs and CD38. Tissue NAD+ declines with age in animal and human studies, and restoring it is one of the most actively researched questions in metabolic and aging biology. Direct NAD+ is studied at hundreds of milligrams per administration, which is the weight it holds in this vial.
MOTS-c is a 16-amino-acid peptide encoded in the 12S rRNA region of mitochondrial DNA, one of the mitochondrial-derived peptides. In rodent models it activates AMPK, shifts the folate and methionine cycles, and improves glucose handling and exercise capacity, which earned it the exercise-mimetic label. Circulating MOTS-c falls with age in human cohorts. It is the signal in this blend: the compound that tells the cell to spend its NAD+ on metabolic work.
5-Amino-1MQ is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), the enzyme that methylates nicotinamide, the NAD+ precursor the salvage pathway would otherwise recycle, and sends it out of the body. Blocking NNMT in diet-induced obese mice raised NAD+ in adipose tissue and reduced fat mass without a change in intake. It is the guard in this blend: the compound that keeps the NAD+ pool from leaking.
Three compounds means three lines to read. A blend COA is only useful if it resolves each component, so ours is run as a component test: NAD+, MOTS-c and 5-Amino-1MQ each report their own peak and their own share of the vial, and the 500 / 20 / 20 split is documented rather than assumed from the label. The certificate for the 100/10/10 on its own page shows what that looks like, with all three weighed.
Check the milligram figure on each line against the label. The two 20mg lines are the ones most vendors skip on a blend this NAD+-heavy, so read those first.
Fill the pool, guard the pool, put the pool to work. Three angles on the same NAD+ system.
The original. A MOTS-c and 5-Amino-1MQ blend with a little NAD+ on top. Choose it for a MOTS-c and NNMT question where NAD+ is being supplied another way or is not the point. Shop the 100/10/10 →
The version where all three lead. Choose it for a question about the NAD+ system as a whole, where the coenzyme, the guard and the signal all need to be present at real weight from one vial.
Twice the NAD+ of IGNITE-540 with no MOTS-c or 5-Amino-1MQ. Choose it for a pure NAD+ question, or to run the leads from their own singles at a ratio of your choosing. Shop NAD+ 1000mg →
What's in the Vial
One lyophilized vial containing 540mg of blended material: 500mg NAD+, 20mg MOTS-c and 20mg 5-Amino-1MQ, supplied for laboratory research use. Reconstitute with bacteriostatic water. Component-tested for purity by third-party HPLC analysis. COA available on this product page.
- NAD+ at 500mg, inside the range it is studied at on its own, not a tenth of it
- MOTS-c and 5-Amino-1MQ doubled to 20mg each, so the leads were not traded for the NAD+
- Three compounds on one NAD+ system: the pool, the guard on the pool, the signal that spends it
- One vial, one reconstitution, one draw instead of five vials and a leftover half of NAD+
- $120 against $165 for the same material as singles, and $72 a vial in a 10-pack
- Every component weighed on the certificate, three lines, not a total
- Built from the 100/10/10 our customers already buy, with the one number that was off corrected
Research Context
NAD+ biology is one of the most studied areas in metabolic and aging research, with human trials on NAD+ precursors and a smaller body of work on NAD+ given directly. MOTS-c has a deep rodent literature on glucose handling, exercise capacity and age-related metabolic decline, and limited early human data. 5-Amino-1MQ has been studied in cell and mouse models of NNMT inhibition and adiposity. None of the three is FDA-approved for human use, and there is no published research on the three as a combination. The blend is offered for researchers studying the NAD+ system who want the coenzyme, an NNMT inhibitor and a mitochondrial signaling peptide in one documented vial. Researchers working with it are ahead of the formal evidence base.
Stacking Notes
The blend is itself a stack, and it covers the NAD+ side of mitochondrial research. Researchers interested in the structural side of the organelle pair it with SS-31, which targets cardiolipin in the inner membrane, either as the single or as MitoStack, where SS-31 and MOTS-c are blended 25 and 25. Running IGNITE-540 next to MitoStack doubles up on MOTS-c, so account for that when planning amounts. No published data exists on these combinations.
Storage
Store the lyophilized product refrigerated and away from light. Keep sealed and protected from moisture until use. After reconstitution, keep refrigerated and use within the period indicated on the product label. Do not freeze.
- 540mg per vial
- 500mg NAD+ · 20mg MOTS-c · 20mg 5-Amino-1MQ
- 25:1:1, up from the 100/10/10's 10:1:1
- Five times the NAD+, double the leads
- $120 per vial, $0.22/mg
- 10-pack: $72 per vial, $0.13/mg
- Triple metabolic research blend on one NAD+ system
- Single reconstitution, single draw
- Component-tested, third-party HPLC
- COA available on product page
- Not FDA approved
- Lyophilized for shelf stability
